ISIS Neutron and Muon Source Data Journal

Effect of cations on bacterial lipopolysaccharides (LPS) self-assembled structures

Abstract: Lipopolysaccharides (LPS) is a key structural component of the outer membrane of gram-negative bacteria. LPS can trigger lethal septic shocks when released into a host?s bloodstream (i.e. the endotoxic effect). Cations can complex with LPS and lead to LPS aggregates structure transformation, which affects bacterial membrane integrity and LPS virulence. Here, we propose a small-angle neutron scattering study (SANS) of self-assembled structures formed by different LPS chemotypes in solutions of different cations at near physiological conditions. We will vary the cation valency (comparing monovalent (Na+), divalent (Ca2+) and multivalent cations (La3+)), as well as the LPS architecture, i.e. its carbohydrate head group length (LPS-smooth, LPS-Ra and LPS-Rd). We also propose to investigate the temperature effect on these systems.

Principal Investigator: Professor Wuge Briscoe
Experimenter: Miss Lauren Matthews
Experimenter: Miss Xueying Guo
Experimenter: Mr Gang Wang
Local Contact: Dr Leide Cavalcanti
Experimenter: Dr Bhaveshkumar Narendrabhai Bharatiya

DOI: 10.5286/ISIS.E.RB2010218

ISIS Experiment Number: RB2010218

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Wuge Briscoe et al; (2021): Effect of cations on bacterial lipopolysaccharides (LPS) self-assembled structures, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010218