ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Lipid dynamics in yeast cell membranes – effect of ergosterol and its precursors
Abstract: A cell membrane can contain hundreds of different lipid molecules, but our understanding of the dynamics of cell membranes is very limited. As a result of a collaboration between the ILL and ESS on the deuteration of lipids from cell cultures, we can now produce 100s of milligrams of lipid extracts from yeast and other microbial cell cultures, sufficient for neutron spectroscopy studies of their dynamics. We recently carried out the first QENS experiment on IRIS to probe the dynamics of the phospholipid extract from P. pastoris yeast, and found that there is a significant difference in their average molecular dynamics compared to model lipids such as POPC and DMPC. We now propose to continue our investigation on IRIS to probe the effect of ergosterol and its drug-resistance related precursors on yeast phospholipid dynamics.
Principal Investigator: Dr Hanna Wacklin-Knecht
Local Contact: Dr Victoria Garcia Sakai
Experimenter: Mr Juan Manuel Orozco Rodriguez
Experimenter: Dr Wolfgang Knecht
DOI: 10.5286/ISIS.E.RB1920364
ISIS Experiment Number: RB1920364
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB1920364-1 | IRIS | 22 November 2022 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Hanna Wacklin-Knecht et al; (2019): Lipid dynamics in yeast cell membranes – effect of ergosterol and its precursors, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920364
