ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Dynamics of amyloid protein fibril growth using isotope-labelled SANS
Abstract: This proposal aims use a novel approach whereby deuterated amyloid peptide fibrils are givenhydrogenated growing ends, and analysed using SANS. The data will enable us to measureindependently both fibril length and linear growth rates for amyloid fibrils. This cannot be doneusing current biophysical techniques, which only probe the rates of conversion of overall %peptide in non-fibrillar to fibrillar form; our proposed experiments absolutely depend on the ability to exploit the contrast between hydrogenated and deuterated peptides that neutron scattering allows. Linear growth rate information is key to understanding the physico-chemical and biological properties of amyloid fibrils, in processes ranging from prion disease propagation, to self-assembly of artificial peptide nano-fibers, and our method will greatly enhance research in the field.
Public release date: 29 October 2014
Principal Investigator: Dr Adam Squires
Experimenter: Dr Cameron Neylon
Experimenter: Ms Gudrun Lotze
Experimenter: Professor Annela Seddon
DOI: 10.5286/ISIS.E.24088071
ISIS Experiment Number: RB1110422
Part Number: 1 - SANS2D
Date of Experiment: 28 October 2011
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Adam Squires et al; (2011): Dynamics of amyloid protein fibril growth using isotope-labelled SANS, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.24088071
