ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Probing the effect of protein corona on the interaction of miRNA-loaded fluorinated dendrimer with model membrane
Abstract: Gene alteration is responsible for several complex illnesses. Gene therapy, working on altering, modifying, or silencing defective or mutated genes, has emerged as a promising therapeutic strategy. Dendritic-based vectors are good candidates for gene delivery due to their well-defined chemical structure, easy tunability of density of terminal groups, and easy surface modification. The addition of fluorinated chains enhances serum stability of dendriplexes (complexes between dendritic vectors and genes), facilitates endosomal escape, enhances cellular uptake and reduces cytotoxicity. We combined Bis-MPA-based dendrimers with fluorination to improve their efficacy as gene vectors. We selected branched fluorinated moiety bearing three perfluoro-t-butoxyl groups as hydrophobic part and synthetized a fluorinated Janus-type dendrimer (FJD2N). The presence of four primary ammonium groups allows complexation with nucleic acids and has the potential to enhance cellular uptake and endosomal escape. We aim to understand the effect of protein corona on the interaction of miRNA-FJD2N with cellular model membranes for the development of more efficient gene delivery systems.
Principal Investigator: Dr Francesca Baldelli Bombelli
Experimenter: Ms Flavia Borrelli
Local Contact: Dr Mario Campana
Experimenter: Dr Federica Sebastiani
Experimenter: Mrs Martina Beccalli
DOI: 10.5286/ISIS.E.RB2500056
ISIS Experiment Number: RB2500056
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2500056-1 | INTER | 19 October 2029 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Francesca Baldelli Bombelli et al; (2026): Probing the effect of protein corona on the interaction of miRNA-loaded fluorinated dendrimer with model membrane, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2500056
Data is released under the CC-BY-4.0 license.
