ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Probing enzyme-induced structural reorganization in lipid nanoparticles
Abstract: Phospholipase D (PLD) is a key enzyme in humans, cleaving the headgroup of phosphatidylcholine lipids to generate phosphatidic acid, thereby regulating lipid metabolism in cells. PLD is also overexpressed in certain cancer types, and in regions of the cell (endosomes) where drug delivery takes place. Our group is investigating the impact of lipid nanoparticle (LNP) structure on PLD activity to engineer nanoparticles to selectively release their cargo. A key challenge in such structure function studies is to decouple the effects of nanoparticle structure and composition, since the structure is often tuned by altering the lipid composition. Recent work demonstrated that stable LNPs of inverse hexagonal (HII) and inverse micellar (Fd3m) phases can be obtained by tuning the ratio of lipid components. This enables us to study how PLD interacts with different LNPs sharing the same lipids - but different molar ratios - and how this interaction drives lipid reorganization within the LNPs for the purpose of exploiting this for drug delivery. The key goal of this experiment is to investigate how PLD activity on LNPs impacts their structure and re-arrangement of their lipid components. Understanding how PLD impacts lipid reorganization in LNPs is key for engineering enzyme responsive LNPs for delivery.
Principal Investigator: Dr Hanna Barriga
Experimenter: Miss Elin Palm
Experimenter: Ms Anna Carlotta Grundel
Local Contact: Dr James Doutch
Experimenter: Dr Marco Fornasier
DOI: 10.5286/ISIS.E.RB2610644
ISIS Experiment Number: RB2610644
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2610644-1 | SANS2D | 14 October 2029 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Hanna Barriga et al; (2026): Probing enzyme-induced structural reorganization in lipid nanoparticles, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2610644
Data is released under the CC-BY-4.0 license.
