ISIS Neutron and Muon Source Data Journal

Understanding molecular interactions between Dengue virus capsid protein (DENV-C) and cellular lipid droplets (LDs): A SANS study

Abstract: We have performed trial SANS and XRR measurements in response to helpful comments from reviewres (Fig.2 and Fig.3). Dengue virus (DENV) is the cause of mosquito-borne dengue fever. Endemic in 100 countries, it leads to over 400 million infections and 25k deaths annually worldwide. With no effective treatments or vaccines, it represents a major socio-economic burden for tropical and subtropical developing countries. Expansion of dengue Aedes mosquito vector due to climate change makes it increasingly a global threat, with over 4 billion people at risk, including EU and UK. There is an urgent need for detailed understanding of the DENV lifecycle for tackling DENV infections. Central to DENV s lifecycle is its replication, a process that has been very recently linked with lipid droplets (LD; ubiquitous multifunctional intracellular organelles of 0.05-100 um in size), with DENV capsid proteins (DENV-C) found accumulating on LD, leading to nucleocapsid formation and viral particle self-assembly. Critically, this process is initiated by the adsorption of DENV-C onto the LD surface. As part of a recently funded EPSRC (UK) SFI (Ireland) project to understand such molecular interactions, here we propose a SANS study of artificial lipid droplets (ALDs) and their interactions with DENV-C proteins, correlating the protein sequence with the ALD-peptide complex nanostructure. The results will underpin future vaccine development strategies exploiting suppressing DENV-C adsorption on LDs.

Principal Investigator: Professor Wuge Briscoe
Experimenter: Dr Zeyuan Zhuang
Experimenter: Mr Guangzhen Wang
Experimenter: Miss Holly Stockdale
Experimenter: Dr Peixun Li
Experimenter: Miss Hongye Li
Local Contact: Dr Leide Cavalcanti
Experimenter: Miss Jia He
Experimenter: Dr Sarah Rogers
Experimenter: Professor Jian Lu
Experimenter: Mr Haoran Li

DOI: 10.5286/ISIS.E.RB2610600

ISIS Experiment Number: RB2610600

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Wuge Briscoe et al; (2026): Understanding molecular interactions between Dengue virus capsid protein (DENV-C) and cellular lipid droplets (LDs): A SANS study, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2610600

Data is released under the CC-BY-4.0 license.