ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Mesoscale structural examination of a stapled homodimer enzyme hydrogel: determining the impact of network formation on force-induced enzyme dissociation
Abstract: We recently created all-enzyme hydrogels by photochemically cross-linking the enzyme glucose oxidase (GluOx). We demonstrated that wild type GluOx hydrogels remain catalytic and are promising biomaterials for biomedical applications. However, as GluOx is homodimeric, there is evidence that mechanical dissociation occurs due to rapid gelation. Recent steered molecular dynamics simulations suggest that dissociation likely proceeds more rapidly than unfolding. To optimise hydrogel catalytic activity, force induced dissociation must be minimised. We have employed a separate chemical cross-linking approach to staple the dimer together and minimise dissociation. To provide crucial validation, this proposal aims to understand both the mesoscale hydrogel structure and the shape and size of the stapled GluOx upon gelation to aid in rational material design. We propose to use SANS to obtain structural information and quantify the degree of dissociation in hydrogels. This information will reveal the proportion of enzymes which remain associated and active following gelation, and their substrate accessibility, giving fundamental insight into catalysis of these new biomaterials.
Principal Investigator: Professor Lorna Dougan
Experimenter: Dr Matthew Hughes
Local Contact: Dr Najet Mahmoudi
Experimenter: Dr Harrison Laurent
DOI: 10.5286/ISIS.E.RB2610200
ISIS Experiment Number: RB2610200
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2610200-1 | SANS2D | 26 May 2029 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Professor Lorna Dougan et al; (2026): Mesoscale structural examination of a stapled homodimer enzyme hydrogel: determining the impact of network formation on force-induced enzyme dissociation, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2610200
Data is released under the CC-BY-4.0 license.
