ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Unraveling the role of Myelin Basic Protein in maintaining membrane asymmetry in the Myelin sheath
Abstract: Myelin constitutes the membrane material surrounding axons and consists of alternating extracellular and cytoplasmic leaflets, where there is distinct asymmetry in the lipid composition of each leaflet. The bilayers are in close contact providing a low dielectric constant through the compact bilayers, which is essential for efficient and fast saltatory propagation of nerve impulses. Structural changes of the myelin sheath are signatures of several inflammatory neurological disorders. The Myelin Basic Protein (MBP) is one of the main proteins in myelin and plays an essential role in stabilising and maintaining the myelin structure. It is located only between the 2 cytoplasmic faces, where it acts as an intermembrane adhesion protein to maintain the close contact between membranes in the myelin sheath. Our previous experiments at ISIS have revealed that membrane asymmetry of supported Myelin bilayers is lost upon heating the membrane to physiological temperatures, and that this asymmetry is essential for efficient MBP interaction. Here, we propose to utilise neutron reflectometry and its unique sensitivity to differentially isotopically labelled asymmetric SMBs to investigate the role that MBP itself has in maintaining the the asymmetry of the myelin membranes at physiological temperatures, and the role of cytoplasmic-facing anionic lipids in MBP recruitment.
Principal Investigator: Dr Julio Martín Pusterla
Experimenter: Dr Andreas Stadler
Local Contact: Dr Stephen Hall
Experimenter: Ms Karoline Rengel
DOI: 10.5286/ISIS.E.RB2610652
ISIS Experiment Number: RB2610652
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2610652-1 | INTER | 30 May 2029 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Julio Martín Pusterla et al; (2026): Unraveling the role of Myelin Basic Protein in maintaining membrane asymmetry in the Myelin sheath, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2610652
Data is released under the CC-BY-4.0 license.
