ISIS Neutron and Muon Source Data Journal

G-quadruplexes' chemical heterogeneity at the nanoscale probed by SANS

Abstract: Aqueous solutions of guanosine-5-monophosphates (GMP) ? known to form G-quadruplexes made of stacked quartet ? can be induced to turn into high water content hydrogels by the addition of guanosine (Gua). A combination of SAXS, Light Scattering and AFM suggested that Gua/GMP hydrogels are microscopically heterogeneous, formed by Gua-rich disordered microcoils of intertwined flexible filaments (??knots??) connected by GMP-rich long linear threads. To best design the characteristics of the hydrogel depending on the desired biomedical or biotech application, an accurate insight into the molecular structure of the 3D G-hydrogel needs to assess the presence of chemical heterogeneity at the nanoscale. We then propose to perform contrast variation SANS experiments as a key technique for deriving the distribution of the Gua and GMP components in the G-quadruplexes.

Principal Investigator: Dr Alessia Pepe
Principal Investigator: Dr Alessia Pepe
Local Contact: Dr Najet Mahmoudi
Experimenter: Professor Paolo Mariani
Experimenter: Dr Francesca Ripanti
Experimenter: Professor Francesco Spinozzi
Experimenter: Dr Paolo Moretti
Experimenter: Miss Carola Nardin
Experimenter: Miss Matilde Montenovo
Local Contact: Dr James Doutch

DOI: 10.5286/ISIS.E.RB2520384

ISIS Experiment Number: RB2520384

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Alessia Pepe et al; (2026): G-quadruplexes' chemical heterogeneity at the nanoscale probed by SANS, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2520384

Data is released under the CC-BY-4.0 license.