ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
mRNA-lipid nanoparticles with distinctly internal structures
Abstract: Lipid nanoparticles (LNPs) are a powerful delivery platform for nucleic acid-based therapeutics. These formulations normally consist of four main components: an ionizable amino lipid, a helper lipid, cholesterol, and a polyethylene glycol (PEG)-lipid conjugate. LNPs can have an intricate internal structure, including liquid crystalline phases such as cubic, hexagonal and lamellar. The internal structure adopted by the LNPs are dictated by the lipid composition and significantly influence the efficiency of mRNA delivery. We have developed LNPs can adopt cubic phases when the helper lipid is replaced with a positively charged variant, particularly at elevated concentrations. This modification improved the RNA transfection of the particles in typically hard-to-transfect bone marrow-derived macrophages. In this work, we want to study the effect of composition on structure and also the partition of different lipids in the core and shell of the LNPs. For that, we plan to use SANS with deuterated lipids and different ratios of D2O/H2O as background. The findings from this study could help the rational design of LNPs with tailored nanostructures and benefit the development of advanced nucleic acid-based therapeutics.
Principal Investigator: Dr Dennis Aschmann
Experimenter: Mr Oscar Escalona Rayo
Experimenter: Dr Ilja Voets
Experimenter: Mr Rafael Vasconcelos de Melo Freire
Local Contact: Dr Gregory Smith
DOI: 10.5286/ISIS.E.RB2569103
ISIS Experiment Number: RB2569103
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2569103-1 | LARMOR | 28 February 2029 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Dennis Aschmann et al; (2026): mRNA-lipid nanoparticles with distinctly internal structures , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2569103
Data is released under the CC-BY-4.0 license.
