ISIS Neutron and Muon Source Data Journal

Rational Design of Broad-Spectrum Antimicrobial Peptides Derived from the Dengue Virus Capsid Alpha2 Sequence

Abstract: Antimicrobial peptides (AMP) offer an attractive alternative to antibiotics in the global fight against antibiotic resistance. This work seeks to design a group of amphiphilic AMPs based on the ?2 sequence of a Dengue viral capsid protein to address the challenge of ineffective membrane disruptions of AMPs. The design was also inspired by the well-studied G(IIKK)3I-NH2 (G3) for broad-spectrum antimicrobial actions. Because the ?2 sequence of the DENV2 C protein is predominantly hydrophobic, it is thought to play the leading role in its own dimerization and membrane insertion during viral infection. Following the native ?2 sequence, three Dengue viral-inspired peptides (DVP 1-3), were designed by rebalancing their amphiphilicity and cationic charges. Modified amino acids to enhance hydrophilicity and cationicity (DVP-1). Further sequence modifications involve swapping L with I to reduce toxicity (DVP-2) and swapping K with R to evaluate antimicrobial efficacy and toxicity (DVP-3). This study systematically investigates how key amino acids and the interplay between hydrophilic and hydrophobic sequences influence the interaction of short peptides with bacterial. SANS allows structural determination associated with interfacial interactions at the molecular level. When combined with deuterium labelling, it can determine how virus protein binds to the lipid SUVs through resolving runs measured under parallel isotopic contrasts.

Principal Investigator: Miss Yingyu Wang
Experimenter: Dr Zeyuan Zhuang
Experimenter: Ms Ziqing Gong
Experimenter: Professor Jian Lu
Experimenter: Miss Caroline Ding
Local Contact: Dr Dirk Honecker

DOI: 10.5286/ISIS.E.RB2520467

ISIS Experiment Number: RB2520467

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Miss Yingyu Wang et al; (2026): Rational Design of Broad-Spectrum Antimicrobial Peptides Derived from the Dengue Virus Capsid Alpha2 Sequence , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2520467

Data is released under the CC-BY-4.0 license.