ISIS Neutron and Muon Source Data Journal

Probing the interaction of liposomal delivery systems with model Gram-negative bacterial cell envelopes

Abstract: Antimicrobial resistance (AMR) is a major threat to global public health, particularly with respect to Gram-negative bacteria, for which the resistance is largely a result of their complex double-membrane envelope structure. Liposomes can effectively deliver anti-infectives across the Gram-negative cell envelope, however, the mechanism of liposome-cell envelope membrane interactions is not fully understood. As part of a larger study aiming to characterise the ability of a selection of liposomal formulations to interact with Gram-negative bacteria possessing different cell envelope structures, we propose to perform neutron reflectometry with isotopic contrast variation to investigate the adhesion/fusion or exchange of lipids during the interaction of liposomes with models of the Gram-negative outer membrane (OM) outer leaflet at air/water interface. The results of this work will be invaluable in understanding the interaction of liposomal membranes with the OM of Gram-negative bacteria, and in the improved design of liposomes for the delivery of anti-infective agents.

Principal Investigator: Dr Yixuan Yan
Local Contact: Dr Maxmilian Skoda
Experimenter: Dr Sarah Gordon
Experimenter: Professor Jayne Lawrence

DOI: 10.5286/ISIS.E.RB2520346

ISIS Experiment Number: RB2520346

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Yixuan Yan et al; (2025): Probing the interaction of liposomal delivery systems with model Gram-negative bacterial cell envelopes , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2520346

Data is released under the CC-BY-4.0 license.