ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Investigating EnB1-Induced Membrane Remodeling via Contrast-Variation Neutron Scattering
Abstract: Cellular membranes exhibit complex three-dimensional architectures essential for numerous biological processes. Membrane-binding proteins contribute to shaping these structures by inducing curvature and remodeling lipid bilayers into tubules and vesicles. Among them, proteins from the Bin/Amphiphysin/Rvs167 (BAR) domain superfamily are key players. These peripheral dimers possess a conserved coiled-coil domain that forms a banana-shaped BAR domain capable of sensing and generating curvature. N-BAR proteins, a subclass, also contain N-terminal amphipathic helices that insert into membranes to enhance curvature. Endophilin B1 (EnB1), an N-BAR protein, participates in oncogenesis-related pathways, including autophagy and apoptosis. Its amphipathic regions mediate membrane association and tubule formation. Cryo-EM has shown EnB1 decorates lipid vesicles and binds nanodiscs as dimers, but these structures do not fully resolve lipid bilayer rearrangements. To address this, we propose using contrast variation small-angle neutron scattering (SANS), which enables selective visualization of sample components by modulating the D?O/H?O ratio. Unlike X-rays, neutrons scatter based on nuclear scattering length density, allowing specific masking of components such as protein, lipid tails, or headgroups. This approach will enable detailed analysis of lipid bilayer remodeling induced by EnB1.
Principal Investigator: Dr Anna Sundborger-Lunna
Experimenter: Mr Arni Thorlacius
Experimenter: Dr Maksim Rulev
Local Contact: Dr Najet Mahmoudi
DOI: 10.5286/ISIS.E.RB2520439
ISIS Experiment Number: RB2520439
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2520439-1 | SANS2D | 17 December 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Anna Sundborger-Lunna et al; (2025): Investigating EnB1-Induced Membrane Remodeling via Contrast-Variation Neutron Scattering, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2520439
Data is released under the CC-BY-4.0 license.
