ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Quantifying lipid exchage in bulk solution with protein-containing SMALP nanodiscs
Abstract: Poly-Styrene-co-Maleic Acid Lipid Particles (SMALPs) are discoidal self-assembled nanoparticles containing a central lipid bilayer core stabilised by a radial amphipathic polymer belt, used to extract membrane proteins directly from cell membranes complete with annular lipids. Recent studies have shown that lipids within SMALPs rapidly exchange with other SMALPs in bulk solution as well as interfacial lipid mono/bilayers. However, this contradicts the assumption that the native lipid environment of a SMALP-encapsulated protein is maintained. To date, it is unknown what influence the presence of a protein within the SMALP has on the propensity for lipids to exchange, which is vital to understand in order to fully realise the biotechnological potential of SMALPs. We have investigated lipid exchange in protein-containing SMALPs with solid-supported bilayers using neutron reflectometry. However, this does not give information on the fate of the nanodiscs themselves. Here, we propose to use SANS with a combination of static structural measurements and time-resolved zero-contrast match point experiments to determine the rate and extent of lipid exchange between protein-containing SMALPs and large unilamellar vesicles in bulk solution, and determine the structural and compositional effect on exchange processes on the SMALPs themselves.
Principal Investigator: Dr Stephen Hall
Experimenter: Dr Naomi Pollock
Experimenter: Professor Karen Edler
Local Contact: Dr Najet Mahmoudi
Experimenter: Professor Tim Dafforn
Experimenter: Dr Thomas Arnold
Experimenter: Dr Andrew Quigley
DOI: 10.5286/ISIS.E.RB2510739
ISIS Experiment Number: RB2510739
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2510739-1 | SANS2D | 31 July 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Stephen Hall et al; (2025): Quantifying lipid exchage in bulk solution with protein-containing SMALP nanodiscs, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2510739
Data is released under the CC-BY-4.0 license.
