ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Developing an Assay to Monitor Neuronal Ceroid Lipofuscinoses Type 7 Flipping Activity in the Lipid Matrix
Abstract: Neuronal Ceroid Lipofuscinoses Type 7 (CLN7) is a Transporter localised to the lysozyme. Genetic mutations associated with CLN7 lead to Neuronal Ceroid Lipofuscinosis. The function of CLN7 is also poorly characterised. Our collaborators recently obtained some highly indicative data from the proteomic analysis of CLN7 knockout mouse models that suggests lysophosphoglycerol (LPG) and/or phosphoglycerol (PG) as a likely candidates for transport by CLN7. We require a direct biochemical assay confirming lipid flipping of either LPG or PG to support a publication and thus propose to use Neutron reflectometry on samples of CLN7 reinstituted into supported lipid bilayers (SLBs) to track the flipping of PG or LPG from one side of the membrane to the other.
Principal Investigator: Dr Andrew Quigley
Local Contact: Dr Luke Clifton
Experimenter: Dr Peter Harrison
Experimenter: Miss Tereza Vecerkova
DOI: 10.5286/ISIS.E.RB2510696
ISIS Experiment Number: RB2510696
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2510696-2 | INTER | 18 September 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
Select the data format above to find
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Andrew Quigley et al; (2025): Developing an Assay to Monitor Neuronal Ceroid Lipofuscinoses Type 7 Flipping Activity in the Lipid Matrix, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2510696
Data is released under the CC-BY-4.0 license.
