ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
From macro to micro: How does Pluronic disrupt cell membrane?
Abstract: Although most Pluronic triblocks are non-toxic to human cells, some still cause observable cell death under certain circumstances, which may evoke severe problems during administration. However, what determines the cytotoxicity of the Pluronic triblocks remains largely unexplored. To comprehensively understand Pluronic's impact on human cells, several models were developed, spanning from macro to micro scales. Techniques such as cell viability assessment, membrane leakage, membrane fluidity, and Quartz crystal microbalance with dissipation (QCM-D) were employed to evaluate their cytotoxicity impact. Our results indicated that the cytotoxicity of some triblocks is caused by their cell membrane-lytic ability. However, in-depth information regarding structural changes in cell membranes induced by Pluronic remains unexplored. Neutron reflection (NR), a high-resolution probing technique, is crucial for this investigation. By utilizing deuterium labelling to lipids and water, NR can trace Pluronic and determine the extent of its distribution across the lipid bilayer. Furthermore, it can elucidate the subsequent effects on the bilayer's structure, providing complementary information on Pluronic-lipid interactions.
Principal Investigator: Professor Jian Lu
Local Contact: Dr Mario Campana
Experimenter: Mr kangcheng shen
Experimenter: Mr Shenghui Tu
Experimenter: Dr Mingrui Liao
DOI: 10.5286/ISIS.E.RB2410585
ISIS Experiment Number: RB2410585
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| - | OFFSPEC | 08 August 2028 | Download |
| 10.5286/ISIS.E.RB2410585-2 | INTER | 23 March 2029 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Professor Jian Lu et al; (2025): From macro to micro: How does Pluronic disrupt cell membrane?, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2410585
Data is released under the CC-BY-4.0 license.
