ISIS Neutron and Muon Source Data Journal

Influence of cholesterol content on the detailed internal structure of LNPs

Abstract: There has been a shift in the pharma industry towards development of medicines acting within the cell, typically in the cytosol, thereby creating demand for advanced delivery vehicles, including lipid nanoparticles (LNPs) that deliver their payload intracellularly. Generally, these vehicles are taken into the cell's endosome via a process known as endocytosis. In order for the therapeutic agent to exert its effect, the vehicle (and its payload) must escape the endosome. Currently only 2% of vehicles are released into the cytosol. This study wishes to determine the effect of the concentration of cholesterol on the detailed internal structure of LNPs using small angle neutron scattering in combination with contrast variation in order to determine how much cholesterol is required for LNP production and in this way develop improved delivery LNPs.

Principal Investigator: Professor Jayne Lawrence
Local Contact: Dr Diego Alba Venero
Experimenter: Dr Dave Barlow
Local Contact: Dr Dirk Honecker
Experimenter: Miss Yining Ma
Experimenter: Dr Xianbing Dai

DOI: 10.5286/ISIS.E.RB2510383

ISIS Experiment Number: RB2510383

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jayne Lawrence et al; (2025): Influence of cholesterol content on the detailed internal structure of LNPs, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2510383

Data is released under the CC-BY-4.0 license.