ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Interaction of non-lamellar liquid-crystalline lipid carpets with model endosomal vesicles.
Abstract: Nucleic acid (NA) therapeutics hold great potential, but their success relies on delivery systems, able to promote effective endosomal escape. Emerging evidence suggests that non-lamellar lipid nanoparticles (NLLNPs), with highly curved bilayers, may enhance endosomal escape. We have developed a microfluidic technique to produce NLLNPs using glycerol monooleate and ionizable lipids, which combine topological activity with pH sensitivity. Preliminary studies show these NLLNPs effectively bind to and destabilize endosomal membranes under acidic conditions, but the mechanism remains unclear. We propose to use Neutron Reflectometry (NR) to investigate the mechanism by which NLLNPs destabilize endosomal membranes as a function of pH. To this end, we will employ innovative solid-supported nanometric ?carpets? of NLLNPs and study their interactions with model endosomal vesicles in real-time using Time-Resolved NR. Leveraging selective deuteration and contrast variation, we aim to resolve structural changes and lipid exchange processes occurring during vesicle binding and fusion with NLLNPs. These insights will inform the design of more efficient lipid nanoparticles for NA delivery.
Principal Investigator: Dr Lucrezia Caselli
Experimenter: Mr Jacopo Cardellini
Experimenter: Ms Valentina Pacciani
Local Contact: Dr Mario Campana
Experimenter: Dr Costanza Montis
Experimenter: Professor Debora Berti
DOI: 10.5286/ISIS.E.RB2510580
ISIS Experiment Number: RB2510580
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2510580-1 | INTER | 19 May 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Lucrezia Caselli et al; (2025): Interaction of non-lamellar liquid-crystalline lipid carpets with model endosomal vesicles., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2510580
Data is released under the CC-BY-4.0 license.
