ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Order from Disorder: A combined SANS and in silico approach to disentangling disordered regions and charge interaction in blood clot formation.
Abstract: Fibrin clots are fascinating hierarchical structures which extend across a wide range of length-scales. The building block of fibrin clots is fibrinogen, an abundant plasma protein that is converted to fibrin by thrombin. Fibrin is highly extensible and demonstrates strain stiffening behaviour, which is essential for stress-distribution in the network. However, the structures of fibrin at the sub-fibre level that determine its unique biomechanical properties are unknown. Of particular interest are the role of the disordered, highly flexible ?C chains and charged interactions of the ? chain in the building block fibrinogen. Our aim is to employ an integrated experimental and modelling approach to gain new insights into the role of disordered regions and charged interactions in fibrin formation, made possible through a combination of recombinant protein engineering and expression, small angle neutron scattering, AlphaFold, coarse grained modelling and atomistic Molecular Dynamics (MD). The SANS data will provide insight into fibrin fibre network and the importance of fibrinogen variants in the formation and mechanical properties of both healthy and prothrombotic clot phenotypes.
Principal Investigator: Professor Lorna Dougan
Local Contact: Dr Najet Mahmoudi
Experimenter: Mr Ahmad Boroumand
Experimenter: Dr Timea Feller
Experimenter: Dr Matthew Hughes
DOI: 10.5286/ISIS.E.RB2510699
ISIS Experiment Number: RB2510699
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2510699-1 | SANS2D | 22 May 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Professor Lorna Dougan et al; (2025): Order from Disorder: A combined SANS and in silico approach to disentangling disordered regions and charge interaction in blood clot formation., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2510699
Data is released under the CC-BY-4.0 license.
