ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Using contrast-variation neutron scattering to investigate tissue specific structural differences in extracellular vesicles
Abstract: Extracellular vesicles (EVs) are nanometric lipid bilayer vesicles released by cells from all organisms, that play a crucial role in intercellular communication. EVs contain many of the molecules of the original cells including RNA, proteins and lipids. Therefore, once they are released into the blood circulation from any specific tissues these differences can be studied to offer a window into the cellular conversations during homeostasis. In addition to their intrinsic communication activity, their physicochemical properties and molecular interactions with different complex biological media make EVs promising candidates as biomarkers and as endogenous drug delivery nanocarriers. There is a growing interest in tracking the source tissues of the EVs to inform about normal physiology and pathology. However, hurdles to realising the full potential of EVs in areas ranging from basic biology to clinical applications; persist due to challenges in EV separation, characterisation and functional studies. The International Society for Extracellular Vesicles recently updated its ?Minimal Information for Studies of Extracellular Vesicles?, providing researchers with basic principles on production, separation and characterisation of EVs from multiple sources. Here we aim to use CV-SANS to investigate the nanostructure and distribution of lipids/proteins/nucleic acids; in EVs of different tissues, to provide analytical parameters to differentiate EVs from different tissue sources.
Principal Investigator: Dr Ryan Pink
Local Contact: Dr Najet Mahmoudi
Experimenter: Ms Airstone Bethy
DOI: 10.5286/ISIS.E.RB2500019
ISIS Experiment Number: RB2500019
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2500019-1 | SANS2D | 29 February 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Ryan Pink et al; (2025): Using contrast-variation neutron scattering to investigate tissue specific structural differences in extracellular vesicles, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2500019
Data is released under the CC-BY-4.0 license.
