ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Elucidating the structure of ?biogenic? cubosomes, containing biological components from Red Blood Cell-derived Extracellular Vesicles.
Abstract: Lipid-based liquid crystalline nanoparticles (LCNPs), such as cubosomes and hexosomes, show great promise for drug delivery due to their structural versatility and high fusogenicity with lipid membranes. Hybridizing LCNPs with extracellular vesicles (EVs) may overcome challenges of synthetic nanoparticles, such as immune responses and lack of tissue targeting. We developed a microfluidic method to integrate red blood cell-derived EVs (RBCEVs) into glycerol monooleate (GMO)-based cubosomes, leading to hybrid systems that combine EVs? targeting abilities with LCNPs? drug-loading capacity. These hybrids demonstrated excellent colloidal stability without steric stabilizers. SAXS revealed that incorporating RBCEVs into the GMO scaffold induces a phase transition from a diamond cubic to a primitive cubic phase. However, SAXS cannot resolve the spatial arrangement of RBCEVs? lipids and proteins, which is critical for their biological and biomedical functions. To address this, we will leverage SANS, in combination with selective deuteration and contrast variation, to map the distribution of RBCEVs within the hybrid system, thereby advancing biogenic LCNPs development for drug delivery.
Principal Investigator: Dr Lucrezia Caselli
Experimenter: Mr Jacopo Cardellini
Local Contact: Dr Najet Mahmoudi
Experimenter: Dr Andrea Zendrini
Experimenter: Professor Debora Berti
Experimenter: Dr Costanza Montis
Experimenter: Ms Valentina Pacciani
DOI: 10.5286/ISIS.E.RB2510522
ISIS Experiment Number: RB2510522
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2510522-1 | SANS2D | 18 May 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Lucrezia Caselli et al; (2025): Elucidating the structure of ?biogenic? cubosomes, containing biological components from Red Blood Cell-derived Extracellular Vesicles., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2510522
Data is released under the CC-BY-4.0 license.
