ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Characterizing lipid nanocarriers (LNCs) for targeting intracellular delivery of siRNA
Abstract: Gene therapy by RNA interference is rapidly developing as a personal field of cancer treatment. Small interfering RNA (siRNA) plays a key role, enabling the targeting of disease-causing proteins. The successful application of siRNA relies heavily on efficient delivery systems. Lipid nanocarriers (LNCs) are used as drug delivery systems. However, tissue-specific targeting remains a key challenge to overcome. In this regard, we aim to design T7 peptide-functionalized liposomes, known for their ability to bind to the human transferrin receptor (hTfR), highly expressed on both the blood-brain barrier and blood?tumor barrier for glioma. A modified T7 peptide was synthesized and the highly selective thiol-maleimide reaction was chosen for further functionalization to DOPE-PEG2000-Mal. DOPE-PEG2000-Mal-T7 will be used as an LNCs substituent, with DPPC, DOTAP and cholesterol. By SANS measurements, exploiting the contrast-matching technique by using hydrogenated and deuterated lipids as components of the LNCs, we aim to understand where DOPE-PEG2000-Mal-T7 is located and the interaction between LNCs and hTfR.
Principal Investigator: Professor Luigi Paduano
Local Contact: Dr Najet Mahmoudi
Experimenter: Mrs Ilaria De Cristofaro
Experimenter: Mr Alessandro Cangiano
Local Contact: Dr Leide Cavalcanti
Experimenter: Dr Noemi Gallucci
DOI: 10.5286/ISIS.E.RB2510388
ISIS Experiment Number: RB2510388
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2510388-1 | SANS2D | 24 March 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Professor Luigi Paduano et al; (2025): Characterizing lipid nanocarriers (LNCs) for targeting intracellular delivery of siRNA, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2510388
Data is released under the CC-BY-4.0 license.
