ISIS Neutron and Muon Source Data Journal

Synergistic interactions between antimicrobial peptides magainin 2 and PGLa

Abstract: We suggest to measure the depth of penetration of two cationic antimicrobial peptides, namely magainin 2 (mag2) and PGLa, added separately and as an equimolar cocktail on single supported lipid bilayers. These peptides are known to exhibit a strong synergetic activity on living organisms but very important questions remain about their structure-function relationship. In particular, we want to know if the interaction between the different molecular systems leads to changes in the bilayer penetration depth. Membranes will be made of lipid mixtures mimicking biological systems (POPE/POPG 3/1, POPC/POPG 3/1 and POPE/POPG 1/3) that will permit to address the question of the role of membrane composition (i.e. POPE vs POPC) and to analyse the effect of anionic lipid concentration. While 25 to 30% of POPG represent well membranes of E. coli much higher lipid content can be encountered with Gram-positive bacterial membranes. 5 days of beamtime on INTER or 6 days on OFFSPEC are requested for this study.

Principal Investigator: Professor Burkhard Bechinger
Local Contact: Dr Mario Campana
Experimenter: Dr Arnaud MARQUETTE

DOI: 10.5286/ISIS.E.RB2410361

ISIS Experiment Number: RB2410361

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Burkhard Bechinger et al; (2025): Synergistic interactions between antimicrobial peptides magainin 2 and PGLa, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2410361

Data is released under the CC-BY-4.0 license.