ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Understanding the interplay between the mammalian plasma membrane lipids and chondroitin sulfate chains
Abstract: The project aims to investigate the structural arrangements of chondroitin sulfate (CS), a carbohydrate present in the extracellular matrix, on the surface of supported lipid bilayers (SLBs) resembling the mammalian plasma membrane. Key components include the role of phospholipids (PC and PS) and cholesterol in these arrangements, particularly in healthy and inflamed cellular states. Neutron reflectometry (NR) is employed to analyze bilayer structure and composition with high resolution. A novel protocol involving Langmuir-Blodgett (LB)/Langmuir-Schaeffer (LS) deposition is proposed to incorporate CS-functionalized lipids into SLBs. Various SLB compositions are tested to mimic different cellular environments, with an emphasis on the influence of lipid composition on CS structural arrangement. Additionally, experiments are conducted at different pH levels to simulate acidic conditions typical of cancer cells. Overall, the study aims to shed light on the interactions between phospholipids and CS variants at the cell membrane interface, with potential implications for understanding cancer cell surface properties and identification.
Principal Investigator: Dr Alessandra Luchini
Experimenter: Mrs Brigida Romano
Local Contact: Dr Mario Campana
Experimenter: Dr Nicoḷ Paracini
Experimenter: Professor Andrea Orecchini
Experimenter: Dr Ilaria Mosca
DOI: 10.5286/ISIS.E.RB2420222
ISIS Experiment Number: RB2420222
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2420222-1 | OFFSPEC | 14 March 2028 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
Select the data format above to find
out more about it.
Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Alessandra Luchini et al; (2025): Understanding the interplay between the mammalian plasma membrane lipids and chondroitin sulfate chains, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2420222
Data is released under the CC-BY-4.0 license.
