ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
On the bacterial outer membranes: the structure of capsular polysaccharides
Abstract: Gram-negative bacteria are a diverse group of microorganisms characterized by their unique cell structure. The cell surface of Gram(-) bacteria is decorated by glycans such as lipopolysaccharides (LPS) and capsular polysaccharides (CPS). CPS is a high-molecular-weight biopolymer synthesized on a phosphatidylglycerol lipid anchor inside the bacteria and then pass across the membrane through pores. It is not known whether the lipid anchor is extracted from the inner membrane and integrated into the outer membrane or whether it remains in the inner membrane as the CPS crosses the cell envelope. However, if the lipid part is incorporated into the outer membrane of the Gram(-) bacterium, it causes a reduction in the thickness of the hydrophobic part of the membrane and an increase in flexibility, compared with systems with only the lipid part of the LPS. For these reasons, thanks to NR measurements, exploiting hydrogenated and dehumidified lipids, it would be possible to understand the structure and the position of CPS in the bacterial membrane and therefore to develop more efficient and specific antimicrobial systems.
Principal Investigator: Professor Luigi Paduano
Local Contact: Dr Mario Campana
Experimenter: Dr Giuseppe Vitiello
Experimenter: Mr Alessandro Cangiano
Experimenter: Dr Noemi Gallucci
Experimenter: Mrs Ilaria De Cristofaro
DOI: 10.5286/ISIS.E.RB2420115
ISIS Experiment Number: RB2420115
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2420115-1 | INTER | 29 November 2027 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Professor Luigi Paduano et al; (2024): On the bacterial outer membranes: the structure of capsular polysaccharides, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2420115
Data is released under the CC-BY-4.0 license.
