ISIS Neutron and Muon Source Data Journal

Neutron Reflection Studies for the Strong Insertion of Sphingosine in Lipid Membrane at the Solid/Liquid Interface

Abstract: Sphingosine, an important component of the sphingolipids, serves as the backbone of the sphingolipids, and works as the signal molecule to regulate cell fate. The incursion of sphingosine into membranes is always accompanied by an increase of membrane permeability, which results in the leakage from the cytosol, and eventually the cell death. The reason why the sphingosine increases the membrane permeability is currently unclear, but structural changes in the membrane after incorporating sphingosine must play an important role. We propose to use neutron reflectivity (NR) combining with deuterium labelling technique to investigate the planar lipid membrane structure at the solid/water interface in the presence of sphingosine with two various hydrophobic chains. This study will provide the basic understanding of the interfacial behaviour of sphingosine and will give constructive support to elucidate how the unique structure of sphingosine affect the membrane.

Principal Investigator: Dr Peixun Li
Experimenter: Dr Zi Wang
Experimenter: Professor Jeffery Penfold
Experimenter: Dr John Webster
Experimenter: Dr Kun Ma
Experimenter: Dr Yao Chen
Experimenter: Dr Luke Clifton
Local Contact: Dr Maxmilian Skoda
Experimenter: Mr Yonghang Mu

DOI: 10.5286/ISIS.E.RB2310666

ISIS Experiment Number: RB2310666

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Peixun Li et al; (2024): Neutron Reflection Studies for the Strong Insertion of Sphingosine in Lipid Membrane at the Solid/Liquid Interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2310666

Data is released under the CC-BY-4.0 license.