ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Probing microfluidic assembly of mRNA encapsulated lipid nanoparticles
Abstract: Messenger RNA medicines enables the treatment of classically undruggable diseases and immunisation against emergent pathogens by administering the genetic sequence encoding for specific proteins or antigens. However naked mRNA is highly susceptible to endogenous nucleases and does not associate with cells, hence must be formulated. The most translatable approach is to employ small (50-150 nm diameter) lipid nanoparticles (LNPs) which protect and facilitate cellular transport of mRNA. The physicochemical nanostructure of the LNPs are critical to their biological activity. Both properties are controlled by the manufacture parameters used for the assembly process. Yet little is understood about the evolution of the nanostructure within these complex nanoparticles during assembly, which influences their final form and biological efficacy. Hence in this proposal we aim to use neutron scattering via SANS2D to assess the evolution of LNP nanostructure in microfluidic assembly. We will collect the scattering pattern over different distances away from the mixing junction on a quartz microfluidic mixer. Experiments will be conducted under a range of contrast matching regimes to evaluate the assembly dynamics of the mRNA, lipids and total material independently. The outcomes of this experiment will establish a novel protocol to assess LNP assembly and future design of LNP chemistry and manufacture.
Principal Investigator: Dr Pratik Gurnani
Local Contact: Dr Najet Mahmoudi
Experimenter: Dr Naoto Hori
Experimenter: Dr Michael Cook
Experimenter: Miss Nga Man Cheng
Experimenter: Dr Benjamin Fiedler
Experimenter: Mr Alexander Kerr
Experimenter: Dr Abhishek Rajbanshi
DOI: 10.5286/ISIS.E.RB2410377
ISIS Experiment Number: RB2410377
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2410377-1 | SANS2D | 10 August 2027 | Download |
| 10.5286/ISIS.E/ISIS.E.RB2410377-2 | SANS2D | 27 November 2027 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Pratik Gurnani et al; (2024): Probing microfluidic assembly of mRNA encapsulated lipid nanoparticles, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2410377
Data is released under the CC-BY-4.0 license.
