ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
An Asymmetric Protein-Containing Gram-Negative bacterial Outer membrane Model
Abstract: We have been developing advanced planar models of the Gram negative bacterial outer membrane as precise and accurate structural assay systems for antibiotic testing by neutron reflectometry. Here we wish to advance the accuracy and utility of these systems by incorporating integral membrane proteins into the models in the form of porins and depositing the asymmetric membranes through a two stage directed in-situ self-assembly methodology, removing the need for time consuming layer-by-layer deposition. Porin : phospholipid vesicles will be ruptured onto silicon surfaces to produce protein-phospholipid bilayers. Lipopolysaccharides will then be introduced into the bulk media which will exchange the outer leaflet lipids forming a porin:phospholipid:lipopolysaccharide membrane. We intend to control the asymmetry of this construct through bridging the inner leaflet phospholipids to the SiO2 layer based on previous observations by us. Finally we will test the stability of the sample under serum solution and temperature conditions. If successful this study will pave the way for a new level of accuracy in interfacial biomimetics using a much simplified fabrication route. This is a resubmission taking into account the panels previous comments by providing preliminary data in the proposal.
Experimenter: Professor Bart Hoogenboom
Local Contact: Dr Luke Clifton
Experimenter: Miss Corrin Blake
DOI: 10.5286/ISIS.E.RB2410063
ISIS Experiment Number: RB2410063
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2410063-1 | OFFSPEC | 09 March 2027 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Professor Bart Hoogenboom et al; (2024): An Asymmetric Protein-Containing Gram-Negative bacterial Outer membrane Model, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2410063
Data is released under the CC-BY-4.0 license.
