ISIS Neutron and Muon Source Data Journal

This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.


Neutron reflection implication of novel serine-rich antimicrobial peptides killing bacteria

Abstract: Alteration of antimicrobial peptides? (AMPs) relative charge and hydrophobicity affects their self-association, self-assembly and subsequent interaction with bacterial membranes. Herein, we designed novel AMPs containing serine amino acids, to discover how substituting lysine residues for serine influences peptide association and membrane binding compared to a control peptide G(IIKK)3-NH? (G3), with proven antimicrobial potency [1]. AMP antimicrobial abilities are explored with a typical Gram-negative E. coli bacterial inner membrane mimic of POPC/POPG (7:3). Coarse-grained molecular dynamics (MD) simulations reveal peptides with higher serine content tend to form larger aggregates and bind to the membrane as oligomers, forming various intramembrane aggregates. Experimental data from dual polarisation interferometry (DPI) along with MD simulations have placed us in a solid position to undertake neutron reflection (NR) to unravel how these AMPs interact with a model membrane at molecular level.

Experimenter: Mr kangcheng shen
Experimenter: Dr Mingrui Liao
Local Contact: Dr Stephen Hall
Experimenter: Miss Anna Stephens
Experimenter: Miss Tianhao Ge
Experimenter: Professor Jian Lu
Experimenter: Miss Caroline Ding

DOI: 10.5286/ISIS.E.RB2320022

ISIS Experiment Number: RB2320022

Part DOI Instrument Public release date Download Link
10.5286/ISIS.E.RB2320022-1 OFFSPEC 20 November 2026 Download

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Mr kangcheng shen et al; (2023): Neutron reflection implication of novel serine-rich antimicrobial peptides killing bacteria, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2320022

Data is released under the CC-BY-4.0 license.



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