This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Neutron reflection implication of novel serine-rich antimicrobial peptides killing bacteria
Abstract: Alteration of antimicrobial peptides? (AMPs) relative charge and hydrophobicity affects their self-association, self-assembly and subsequent interaction with bacterial membranes. Herein, we designed novel AMPs containing serine amino acids, to discover how substituting lysine residues for serine influences peptide association and membrane binding compared to a control peptide G(IIKK)3-NH? (G3), with proven antimicrobial potency [1]. AMP antimicrobial abilities are explored with a typical Gram-negative E. coli bacterial inner membrane mimic of POPC/POPG (7:3). Coarse-grained molecular dynamics (MD) simulations reveal peptides with higher serine content tend to form larger aggregates and bind to the membrane as oligomers, forming various intramembrane aggregates. Experimental data from dual polarisation interferometry (DPI) along with MD simulations have placed us in a solid position to undertake neutron reflection (NR) to unravel how these AMPs interact with a model membrane at molecular level.
Experimenter: Mr kangcheng shen
Experimenter: Dr Mingrui Liao
Local Contact: Dr Stephen Hall
Experimenter: Miss Anna Stephens
Experimenter: Miss Tianhao Ge
Experimenter: Professor Jian Lu
Experimenter: Miss Caroline Ding
DOI: 10.5286/ISIS.E.RB2320022
ISIS Experiment Number: RB2320022
Part DOI | Instrument | Public release date | Download Link |
---|---|---|---|
10.5286/ISIS.E.RB2320022-1 | OFFSPEC | 20 November 2026 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
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publication is as:
[author], [date], [title], [publisher],
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For Example:
Mr kangcheng shen et al; (2023): Neutron reflection implication of novel serine-rich antimicrobial peptides killing bacteria, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2320022
Data is released under the CC-BY-4.0 license.