ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Effect of LLPS on XoPR/membrane interactions
Abstract: The role of Liquid-liquid phase separation (LLPS) in biology is increasingly recognised. Actin remodelling has been found to involve LLPS, which likely acts to upregulate critical proteins and adjust their activity. Intrinsically disordered protein XopR, has been found to trigger actin remodelling, and undergoes LLPS under a pH of 7.4. Here, neutron reflectometry will be used to determine the consequences of LLPS induction at SLBs by XopR alone in terms of the changes that may occur in the lipid bilayer (SLB restructuring), as well as the type of LLPS that takes place (2D vs 3D, and eventual correlations between LLPS domains. Also, the link between cell membrane stiffness (i.e., cholesterol content) and LLPS at the membrane will be investigated.
Experimenter: Dr Yubexi Correa-Marcano
Experimenter: Dr Claire Buchanan
Local Contact: Dr Luke Clifton
DOI: 10.5286/ISIS.E.RB2320107
ISIS Experiment Number: RB2320107
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2320107-1 | OFFSPEC | 16 October 2026 | Download |
| 10.5286/ISIS.E.RB2320107-2 | OFFSPEC | 22 March 2027 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
Select the data format above to find
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Yubexi Correa-Marcano et al; (2023): Effect of LLPS on XoPR/membrane interactions, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2320107
Data is released under the CC-BY-4.0 license.
