ISIS Neutron and Muon Source Data Journal

How does hydrophilicity change affect adsorption of non-ionic surfactant and monoclonal antibody on PDMS surface?

Abstract: Coating of a layer of polydimethylsiloxane (PDMS) film on the surface of glass or polymeric syringes is widely adopted as the common approach to reduce interfacial friction and avoid syringe jamming. However, the PDMS surface has very different chemical nature from glass or solid plastic substrate and will elicit adsorption of antibody therapeutics, causing structural instability and aggregation. Nonionic surfactants such as Tween 80 (PS80) are widely used to control antibody adsorption, but their exact impact is little explored because many techniques cannot distinguish protein from surfactant at the interface. Following our recent studies of adsorption of PS80 surfactants and antibody onto the PDMS film coated silicon substrate by spectroscopic ellipsometry, we propose a set of neutron reflection studies to determine interfacial structural features that underline how PS80 with different numbers of EO unit can co-adsorb with COE-3 at the PDMS/solution interface.

Local Contact: Dr Mario Campana
Experimenter: Dr Xuzhi Hu
Experimenter: Mr kangcheng shen
Experimenter: Professor Jian Lu
Experimenter: Dr Mingrui Liao

DOI: 10.5286/ISIS.E.RB2310450

ISIS Experiment Number: RB2310450

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Mario Campana et al; (2023): How does hydrophilicity change affect adsorption of non-ionic surfactant and monoclonal antibody on PDMS surface?, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2310450

Data is released under the CC-BY-4.0 license.