ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Determining the membrane location of rationally designed antimicrobial peptides (AMPs) with lipid model membranes (LMMs)__
Abstract: In an effort to thwart the world-wide growing bacterial resistance to current antibiotics, we are studying the structure and function of five novel antimicrobial peptides (AMPs) that are rationally designed using three types of amino acids; we vary peptide chain length, charge, hydrophobicity, hydrophobic moment and secondary structure. These AMPs selectively attack the bacterial membrane rather than a metabolic pathway and so are slower to incur bacterial resistance. We need neutron reflectivity data to precisely locate the peptides in lipid model membranes (LMMs) mimicking Gram-negative and Gram-positive bacteria, and eukaryotic cells. When positional data from NR is combined with membrane thickness data from X-ray diffuse scattering (XDS), we can then compare our structural data to that from molecular dynamics simulators to obtain angstrom-level information about amino acid locations in the membrane. This structural information is required to formulate a hypothesis regarding the mechanism of bacterial killing by AMPs which will guide future AMP synthesis and testing.
Experimenter: Dr Berthony Deslouches
Local Contact: Dr Stephen Hall
Experimenter: Dr Saheli Mitra
Experimenter: Dr Frank Heinrich
Experimenter: Professor Stephanie Tristram-Nagle
DOI: 10.5286/ISIS.E.RB2310027
ISIS Experiment Number: RB2310027
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2310027-1 | OFFSPEC | 15 September 2026 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Berthony Deslouches et al; (2023): Determining the membrane location of rationally designed antimicrobial peptides (AMPs) with lipid model membranes (LMMs)__, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2310027
Data is released under the CC-BY-4.0 license.
