ISIS Neutron and Muon Source Data Journal

Elucidating the interactions between membrane-bound and membraneless organelles using SANS analysis of minimalistic peptide and lipid model systems

Abstract: Cell nucleus contains membrane-bound and membraneless subcellular organelles that accommodate and control biocatalytic processes. Their structures and functions are poorly characterised. Despite recent progress in elucidating the mechanism of membraneless organelles formation by liquid-liquid phase separation1 (LLPS) of intrinsically disordered proteins, the interaction between protein-based organelles and lipid membrane-bound organelles remains unknown. This work follows our recent advances in the models of the two entities using small designed peptides and lipid vesicles, we propose to study how the two model organelles interact across the model interface when they are brought together. The parallel SANS runs under H/D substitutions offer great potential for unraveling the key molecular structural features from representative short peptides and environmental conditions.

Local Contact: Dr James Doutch
Experimenter: Professor Jian Lu
Experimenter: Mr Itai Katzir
Experimenter: Dr Mingrui Liao
Experimenter: Miss Yingyu Wang
Local Contact: Dr Leide Cavalcanti

DOI: 10.5286/ISIS.E.RB2310043

ISIS Experiment Number: RB2310043

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr James Doutch et al; (2023): Elucidating the interactions between membrane-bound and membraneless organelles using SANS analysis of minimalistic peptide and lipid model systems, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2310043

Data is released under the CC-BY-4.0 license.