ISIS Neutron and Muon Source Data Journal

Investigation of the Competitive Adsorption between Monoclonal Antibodies and Non-ionic Surfactants at the Air-Water Interface

Abstract: We aim to gain insight into protein-surfactant complex behaviours at the air-water interface. Specifically, we will investigate two mAbs, supplied by Eli Lilly, with different long-term stabilities and the non-ionic surfactant Poloxamer 188. We propose to investigate the the competitive adsorption between the two components and how it varies based on order of addition and compression/expansion cycling which can represent some conditions seen during formulation and drug administration. This study has high industrial impact and would improve therapeutic formulations to evaluate how well this new surfactant can prevent interfacial mAb aggregation, which can reduce drug efficacy and shelf-life. Neutron reflectivity, alongside a Langmuir trough, can be used to characterize this interface under compression. The experiments will be complemented with other interfacial measurement techniques.

Principal Investigator: Dr Benjamin Thompson
Experimenter: Mr Kiet Pham
Experimenter: Dr Yun Liu
Local Contact: Dr Mario Campana
Local Contact: Dr Oleksandr Tomchuk

DOI: 10.5286/ISIS.E/ISIS.E.RB2220639

ISIS Experiment Number: RB2220639

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Benjamin Thompson et al; (2024): Investigation of the Competitive Adsorption between Monoclonal Antibodies and Non-ionic Surfactants at the Air-Water Interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E/ISIS.E.RB2220639

Data is released under the CC-BY-4.0 license.