ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Effect of Serum Proteins on the Endosomal Interaction of Lipid Nanoparticles
Abstract: Lipid-based nanoparticles (LNPs) have had considerable success as vehicles for delivering mRNA encoding the viral spike protein for protection against COVID-19. To deliver their payload the LNPs have to first enter the cell via the process of endocytosis, protect their payload from the low pH of the endosomal compartment and and then ensure the release of their payload from the endosome. The limited capacity of LNPs to undergo escape from the endosome (<2%) post-cellular uptake is the main barrier to their wider exploitation of LNPs as carriers of other therapeutic agents. Here we propose to use neutron reflectometry in combination with isotopic contrast variation to determine the extent of lipid insertion and exchange between serum coated LNPs (prepared using either of the cationic lipids MC3 or Lipid V) and model early endosomal (EEM) monolayers at a sub-phase pH of 5.5. Understanding the effect of serum proteins on endosomal escape is essential because LNPs will become coated with the proteins prior to endosomal uptake. The results of this study will be important in the design of efficient LNPs that can escape the endosome at an early stage with its payload intact.
Local Contact: Dr Rebecca Welbourn
Experimenter: Professor Jayne Lawrence
Experimenter: Dr Dave Barlow
Experimenter: Dr Vahid Heravi Shargh
Experimenter: Miss Yixuan Yan
DOI: 10.5286/ISIS.E.RB2310503
ISIS Experiment Number: RB2310503
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2310503-1 | INTER | 24 March 2026 | Download |
| 10.5286/ISIS.E.RB2310503-2 | INTER | 26 May 2027 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Rebecca Welbourn et al; (2023): Effect of Serum Proteins on the Endosomal Interaction of Lipid Nanoparticles, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2310503
Data is released under the CC-BY-4.0 license.
