ISIS Neutron and Muon Source Data Journal

Using magnetic reference layers to unravel the interaction of proteins with complex lipid bilayers

Abstract: During a previous experiment (RB1710262), we investigated the structure of sponge-like nanoparticles (LNPs) deposited on a silica surface with and without Aspartic protease (Asp, 34KDa), which is a common food additive in cheese manufacturing. We showed that the structure deposited layer changed when the contrast was changed, and we could fit only data collected from a single contrast, which was not sufficient to properly characterize this system. Here we propose to use substrates including a magnetic reference layer composed of iron, with a thick silica cap to protect the metal from the water. The use of these magnetic substrates will enable us to collect polarized data on the LNP layer, without the need to change liquid contrast, while still getting sufficient data to determine the structure.

Principal Investigator: Professor Tommy Nylander
Experimenter: Professor Björgvin Hjörvarsson
Experimenter: Mr Sebastian Köhler
Experimenter: Professor Sean Langridge
Local Contact: Dr Andrew Caruana
Experimenter: Professor Kenneth Järrendahl
Experimenter: Professor Jens Birch
Experimenter: Mr Ivan Yakymenko
Experimenter: Professor Max Wolff
Experimenter: Dr Alexei Vorobiev
Experimenter: Dr Alessandra Luchini
Experimenter: Dr Jos Cooper

DOI: 10.5286/ISIS.E.RB2220651

ISIS Experiment Number: RB2220651

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Tommy Nylander et al; (2023): Using magnetic reference layers to unravel the interaction of proteins with complex lipid bilayers, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2220651

Data is released under the CC-BY-4.0 license.