ISIS Neutron and Muon Source Data Journal

Interactions between a quadruply-charged X-shaped peptide and bacterial Lipopolysaccharides (LPS) micelles: Complexation or penetration

Abstract: Lipopolysaccharides (LPS) is the main component (~75%) of the Gram-negative bacteria outer membrane which can trigger lethal septic shocks when released into a host?s bloodstream. Oligoglycine tetramer is a cationic four-armed X-shaped synthetic peptide with quadruple charges. The anionic phosphorylated and carboxylated headgroup can be targeted by the X-peptides, conjectured to reduce bacterial virulence via shielding its viral adhesion receptor, as well as affecting the elastic properties and structural integrity of bacterial membranes. We propose a SANS study of the interactions of LPS micelles mediated by the X-peptides, varying the concentration, solution pH and temperature, as well as the LPS architecture. The unprecedented results will underpin understanding of the efficacy of the X-peptide as an antimicrobial peptide in compromising the bacteria membrane structure.

Principal Investigator: Professor Wuge Briscoe
Local Contact: Dr James Doutch
Experimenter: Mr Oliver McDowell
Experimenter: Mr Tao Ma
Experimenter: Dr Magdalena Wlodek
Experimenter: Miss Xueying Guo
Experimenter: Miss Holly Stockdale
Experimenter: Dr Sarah Rogers
Experimenter: Dr Xinhu Wu

DOI: 10.5286/ISIS.E.RB2220122

ISIS Experiment Number: RB2220122

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Wuge Briscoe et al; (2023): Interactions between a quadruply-charged X-shaped peptide and bacterial Lipopolysaccharides (LPS) micelles: Complexation or penetration, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2220122

Data is released under the CC-BY-4.0 license.