ISIS Neutron and Muon Source Data Journal

Long Range Structure and Zwitterion-Ion Interactions in Osmolyte Solutions

Abstract: Cytosol composition not only dictates the osmotic balance of cells with their surroundings, but also determines the the range and nature of inter-protein interactions within the cell. While the role of salt in destabilising proteins is well known, the role that zwitterionic osmolytes play in these interactions is less clear. Here we will study model cytosol solutions of salt-zwitterion mixtures using neutron scattering. Recently we have investigated surface forces present in salt-zwitterion mixtures at an interface, and found periodic structure and long range screening forces that depend on sample composition. This experiment will investigate the occurrence of this structure in the bulk liquid, and its dependence on salt ion species. These results will have significant impact on our understanding of the role of zwitterionic osmolytes in maintaining protein structure and function.

Principal Investigator: Dr James Hallett
Experimenter: Dr Gregory Smith
Local Contact: Dr Terri-Louise Hughes
Experimenter: Mr Kieran Agg
Experimenter: Dr Tom Headen
Experimenter: Professor Susan Perkin
Local Contact: Dr Oliver Alderman
Experimenter: Dr Yin Ka Catherine Fung
Experimenter: Mr Timothy Groves
Experimenter: Dr Shurui Miao

DOI: 10.5286/ISIS.E.RB2220174

ISIS Experiment Number: RB2220174

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr James Hallett et al; (2023): Long Range Structure and Zwitterion-Ion Interactions in Osmolyte Solutions, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2220174

Data is released under the CC-BY-4.0 license.