ISIS Neutron and Muon Source Data Journal

Native-like mitochondrial membrane mimics for apoptosis-regulating proteins

Abstract: Mitochondrial apoptosis is a programmed suicide mechanism in cells essential for life. Upon oxidative stress, the mitochondrial outer membrane (MOM) undergoes permeabilization, followed by the release of apoptotic factors. This process is tightly regulated by the Bcl-2 protein family at the MOM. In healthy cells, pro-apoptitic Bax is neutralized by the anti-apoptotic Bcl-2, but any imbalance in this interaction can cause disorders including cancer. The MOM contains several phospholipid classes, including the mitochondria-specific cardiolipin, and a broad distribution of fatty acyl chains. We are using neutron reflectometry to elucidate the function of the Bcl-2 protein family and in this experiment we will recreate the MOM lipid composition from purified yeast lipid extracts to investigate the binding of Bax to native-like MOM mimics.

Principal Investigator: Dr Hanna Wacklin-Knecht
Local Contact: Dr Luke Clifton
Experimenter: Miss Hannah Burrall
Experimenter: Mr Sebastian Köhler
Experimenter: Dr Jörgen Ådén
Experimenter: Professor Gerhard Groebner
Experimenter: Dr Sophie Ayscough

DOI: 10.5286/ISIS.E.RB2220459

ISIS Experiment Number: RB2220459

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Hanna Wacklin-Knecht et al; (2023): Native-like mitochondrial membrane mimics for apoptosis-regulating proteins, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2220459

Data is released under the CC-BY-4.0 license.