ISIS Neutron and Muon Source Data Journal

NR Study of Co-adsorption of a Monoclonal Antibody and Nonionic Surfactant at the Amphiphilically Modified SiO2/Water Interface

Abstract: From spectroscopic ellipsometry and AFM studies of the adsorption of antibody COE-3 and nonionic surfactant Tween 80 at the UV-treated TMOS layer/water interface, large differences in the adsorbed amount for antibody and surfactant were observed from different adsorption sequences, indicating different impacts of local environments on molecular interactions. This neutron reflection work will help determine how COE-3 and surfactant mix in the adsorbed layer under different sequential adsorption processes. This study constitutes an important part of our systematic efforts to understand how surface chemical nature affects adsorbed mAb structures and causes structural deformation. We require 3 days of beam time for the proposed experiment. Inter is preferred for better structural resolution over the entire Q range and following fast dynamic adsorption processes.

Principal Investigator: Professor Jian Lu
Experimenter: Dr Zongyi Li
Experimenter: Mr kangcheng shen
Experimenter: Miss Anna Stephens
Experimenter: Miss Tianhao Ge
Experimenter: Mr Peter Hollowell
Experimenter: Ms Ilayda Akbulut
Local Contact: Dr Stephen Hall
Experimenter: Mr Zeyuan ZHUANG

DOI: 10.5286/ISIS.E.RB2220154

ISIS Experiment Number: RB2220154

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2023): NR Study of Co-adsorption of a Monoclonal Antibody and Nonionic Surfactant at the Amphiphilically Modified SiO2/Water Interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2220154

Data is released under the CC-BY-4.0 license.