ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Fluorinated Janus-type dendrimer: self-assembly in presence and absence of miRNA
Abstract: Gene alteration is responsible for several complex illnesses. Gene therapy, working on altering, modifying or silencing defective or mutated genes, has emerged as a promising therapeutic strategy. Dendritic-based vectors are a good candidate for gene delivery due to their well-defined chemical structure, easy tunability of density of terminal groups, and ease surface modification. The addition of fluorinated chains enhances serum stability of dendriplexes (complexes between dendritic vectors and genes), facilitates endosomal escape, enhances cellular uptake and reduces cytotoxicity. We combined Bis-MPA-based dendrimers with fluorination to improve their efficacy as gene vectors. We selected branched fluorinated moiety bearing three perfluoro-t-butoxyl groups as hydrophobic part and synthetized a fluorinated Janus-type dendrimer (FJD2N). The presence of four primary ammonium groups allows complexation with nucleic acids. We will characterize the self-assembly of the FJD2N in presence and absence of miRNA, at a range of concentrations and salts conditions. With this knowledge we aim to tune the structural characteristics of dendriplexes in order to control their therapeutic efficacy.
Local Contact: Dr Diego Alba Venero
Experimenter: Dr Marta Rosati
Experimenter: Dr Federica Sebastiani
Experimenter: Dr Stefania Marcuzzo
Experimenter: Dr Francesca Baldelli Bombelli
Local Contact: Dr Najet Mahmoudi
DOI: 10.5286/ISIS.E.RB2310070
ISIS Experiment Number: RB2310070
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB2310070-1 | ZOOM | 24 March 2026 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Diego Alba Venero et al; (2023): Fluorinated Janus-type dendrimer: self-assembly in presence and absence of miRNA, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2310070
Data is released under the CC-BY-4.0 license.
