ISIS Neutron and Muon Source Data Journal

NR Study of Membrane Disruptions by Rationally Designed Cationic Lipopeptides with Short Peptide Sequences

Abstract: A group of designed lipopeptides with only 4 amino acids have displayed large differences in high antimicrobial efficacy against E. coli and high biocompatibility to host cells, as well as in secondary structures upon membrane binding. Neutron reflection is about the only method that could help reveal how these lipopeptides attack inner and outer bacterial membranes from the established membrane models for Gram-negative bacteria, whilst displaying sufficient biocompatibility to host cell membranes. This work is part of PhD research by Tianhao Ge and constitutes an important aspect of our design and development of effective antimicrobial peptides. We request 3 days of INTER time for this study. INTER is preferred for greater structural resolution, wider Q range and also selective runs to establish if any time-dependent binding and structural changes within the timescale of 10 to 60 min.

Principal Investigator: Professor Jian Lu
Experimenter: Mr Zeyuan ZHUANG
Experimenter: Miss Yingyu Wang
Experimenter: Miss Anna Stephens
Experimenter: Dr Arwel Hughes
Experimenter: Miss Tianhao Ge
Local Contact: Dr Stephen Hall
Experimenter: Miss Caroline Ding
Experimenter: Dr Mingrui Liao

DOI: 10.5286/ISIS.E.RB2220145

ISIS Experiment Number: RB2220145

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2022): NR Study of Membrane Disruptions by Rationally Designed Cationic Lipopeptides with Short Peptide Sequences, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2220145

Data is released under the CC-BY-4.0 license.