ISIS Neutron and Muon Source Data Journal

Endosomal Interaction of Lipid Nanoparticles

Abstract: There has been a shift in the pharma industry towards development of medicines containing therapeutic agents acting at sub-cellular compartments, thereby creating a demand for advanced delivery vehicles that convey their payload to the required site of action. A feature of intracellular delivery is that the vehicle is taken up by the target cells via endocytosis with the vehicle and its payload becoming engulfed in the endosome. In order for the therapeutic molecule to exert its effect, it must escape from the endosome. Neutron reflectometry coupled with contrast variation will help to unravel the interaction(s) between lipid nanoparticles (LNPs) carrying RNA and containing different ionisable lipids and the endosomal membrane (particularly the early stage endosomal membrane) to enable the design of efficient LNPs that can escape the endosome at an early stage with an intact RNA payload.

Principal Investigator: Professor Jayne Lawrence
Experimenter: Mr Arslan Siddique
Experimenter: Dr Vahid Heravi Shargh
Local Contact: Dr John Webster
Experimenter: Dr Glenn Coope
Experimenter: Dr Daniel Ondo
Experimenter: Miss Yixuan Yan
Experimenter: Mr Javier Carrascosa Tejedor
Experimenter: Dr Alice Spadea
Experimenter: Dr Richard Campbell
Experimenter: Mr Samuel Winnall

DOI: 10.5286/ISIS.E.RB2220322

ISIS Experiment Number: RB2220322

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jayne Lawrence et al; (2022): Endosomal Interaction of Lipid Nanoparticles, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2220322

Data is released under the CC-BY-4.0 license.