ISIS Neutron and Muon Source Data Journal

NR investigation of the SARS-CoV-2 Spike protein fusion domain interaction with model membranes

Abstract: Coronavirus disease-2019 (COVID-19) is a lethal respiratory illness caused by the coronavirus SARS-CoV-2. A thorough understanding of the molecular mechanisms of cellular infection by coronaviruses is therefore of utmost importance. A critical stage in infection is the fusion between viral and host membranes, which is mediated by the viral Spike Protein. It has now become clear that membrane fusion during viral entry is a cooperative process likely involving the contribution of different sections of the fusion domain, which may also be dependent on the nature and the composition of the lipid mixtures. To address this, we will employ NR to investigate the membrane binding activity of the Fusion domain and Core of the Spike S2 domain, upon interaction with natural lipid bilayers and compare these results with our previous findings involving minimal models with single peptides.

Principal Investigator: Dr Armando Maestro
Local Contact: Dr Stephen Hall
Experimenter: Dr Krishna Batchu
Experimenter: Dr Nathan Zaccai
Experimenter: Dr Giovanna Fragneto
Experimenter: Miss Rosario Moreno Mora
Experimenter: Mr Andreas Santamaria
Experimenter: Dr Nisha Pawar Chauhan

DOI: 10.5286/ISIS.E.RB2210247

ISIS Experiment Number: RB2210247

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Armando Maestro et al; (2022): NR investigation of the SARS-CoV-2 Spike protein fusion domain interaction with model membranes, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210247

Data is released under the CC-BY-4.0 license.