ISIS Neutron and Muon Source Data Journal

Unravelling the solution structures and stability of medically- and industrially-important anti-COVID nanobodies

Abstract: The use of therapeutic antibodies as drugs made by pharmaceutical companies is increasing rapidly (>$75 billion market). Understanding the solution structures and stability of such human antibodies is essential for improving their manufacturing and developing their applications. The hinge disulphides that link the antibody light and heavy chains have a major influence on antibody reactivity. We have recently completed studies of human IgG1, IgG2, IgG3 and IgG4 antibodies, using SAXS, SANS, and analytical ultracentrifugation, plus atomistic modelling. Nanobodies differ from human IgG by only possessing two heavy chains and no light chains, thus offering enhanced epitope binding properties. This continuation project will identify the solution conformation of anti-COVID nanobodies and related molecules by such a combination of methods and their atomistic modelling.

Principal Investigator: Professor Stephen Perkins
Local Contact: Dr James Doutch
Experimenter: Mr xin gao

DOI: 10.5286/ISIS.E.RB2210299

ISIS Experiment Number: RB2210299

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Stephen Perkins et al; (2022): Unravelling the solution structures and stability of medically- and industrially-important anti-COVID nanobodies, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210299

Data is released under the CC-BY-4.0 license.