ISIS Neutron and Muon Source Data Journal

DNA hybridisation-mediated vesicle fusion - kinetics and fate of fusion

Abstract: Fusing vesicles in a controlled manner has major advantages in diagnostic and drug delivery applications, however achieving controlled fusion is not trivial. Commonly used physically mediated fusion techniques such as co-extrusion and freeze-thawing lead to heterogeneous fusion products, where the mixing ratio of different vesicle species is not easily controlled. We have designed a DNA hybridisation-based assay for directed fusion of vesicles upon mixing, and applied it in a recently published diagnostic assay to detect microRNA. In a previous experiment at ZOOM, we studied DNA-functionalised vesicle formulations before and after fusion. Here we will expand this dataset to i) include longer time points to identify when DNA-mediated lipid mixing plateaus, and ii) characterise fusion products with respect to lipid formulation, mixing ratios and DNA density on the vesicle surfaces.

Principal Investigator: Dr Margaret Holme
Local Contact: Dr James Doutch
Experimenter: Dr Hanna Barriga
Experimenter: Professor Molly Stevens
Experimenter: Miss Nayoung Kim
Experimenter: Ms Amy Monahan
Experimenter: Miss Lijun Hu
Experimenter: Miss Peiying Liu
Experimenter: Dr Luke Salter
Experimenter: Dr Miina Ojansivu
Experimenter: Miss Catherine Saunders

DOI: 10.5286/ISIS.E.RB2010544

ISIS Experiment Number: RB2010544

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Margaret Holme et al; (2022): DNA hybridisation-mediated vesicle fusion - kinetics and fate of fusion, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010544

Data is released under the CC-BY-4.0 license.