ISIS Neutron and Muon Source Data Journal

Effects of multivalent cations on bacterial lipopolysaccharides (LPS) monolayer structure

Abstract: Lipopolysaccharides (LPS) is the main component (~75%) of the Gram-negative bacteria outer membrane which can trigger lethal septic shocks when released into a host?s bloodstream. Its anionic phosphorylated and carboxylated headgroup can be targeted by cations which affect the elastic properties and structural integrity of bacterial membranes. However, such interactions are not well understood and in particular there is a gap in your knowledge of the effects of trivalent cations. Such knowledge is of fundamental importance and also of practical implications, as trivalent cations could be exploited to compromise bacterial membrane structures. Here, we propose a Neutron reflectivity (NR) study of the effects of the cations with different valence and ionic radius (Ca2+, Fe3+ and La3+) on LPS monolayers at the LB air-water interface, varying the surface pressure and the temperature.

Principal Investigator: Professor Wuge Briscoe
Experimenter: Professor Jian Lu
Experimenter: Miss Holly Stockdale
Experimenter: Mr Oliver McDowell
Experimenter: Mr Tao Ma
Experimenter: Miss Xueying Guo
Local Contact: Dr Maxmilian Skoda
Experimenter: Mr Michael Stevens
Experimenter: Miss Charlotte Kenton

DOI: 10.5286/ISIS.E.RB2210055

ISIS Experiment Number: RB2210055

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Wuge Briscoe et al; (2022): Effects of multivalent cations on bacterial lipopolysaccharides (LPS) monolayer structure, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210055

Data is released under the CC-BY-4.0 license.