ISIS Neutron and Muon Source Data Journal

Structural studies of integral membrane proteins in Salipro stealth carriers

Abstract: Integral membrane proteins (IMPs) are potential targets for medical applications and are usually extracted with detergent for structural studies. However, SAXS and SANS studies of membrane proteins in detergent micelles are problematic due to the dominating contribution of detergent and empty micelles. Reconstitution of IMPs in Salipro nanoparticles can overcome these obstacles and allow a low-resolution structural analysis of membrane proteins in a detergent free environment. To fully contrast-match out the Salipro nanoparticles, we plan to incorporate our IMP targets in stealth carriers consisting of deuterated Saposin A proteins and lipids and perform a SANS experiment on SANS2D. This would allow a low-resolution structural characterisation of our IMPs of interest in a native-like lipid environment without the often-dominating scattering contribution of the lipid carrier system.

Principal Investigator: Professor Henning Tidow
Experimenter: Professor Trevor Forsyth
Local Contact: Dr Najet Mahmoudi
Experimenter: Dr Sylvain Prévost
Experimenter: Mr Dominique-Maurice Kehlenbeck
Experimenter: Ms Fernanda Alvarado Galindo

DOI: 10.5286/ISIS.E.RB2210042

ISIS Experiment Number: RB2210042

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Henning Tidow et al; (2022): Structural studies of integral membrane proteins in Salipro stealth carriers, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210042

Data is released under the CC-BY-4.0 license.