ISIS Neutron and Muon Source Data Journal

Solvent contrast variation study of DNA nanocarriers based on Transportan peptides

Abstract: Cell-penetrating peptides (CPPs) are short amino acid sequences that can transport molecules or DNA into cells. Delivery of nucleic acids (DNA or RNA) into cells is attracting huge attention as a potential means to treat genetic diseases. We seek to determine the nanostructure of complexes formed between DNA and oppositely charged peptides, based on Transportans, which are a class of CPPs derived from natural sequences of wasp venom proteins and human neuropeptides. We will perform small-angle neutron scattering, using unique capabilities of the Sans2d beamline at ISIS, to investigate the structure of model CPPs in complexes with DNA using contrast variation with mixtures of deuterated and normal water. The scattering length densities of DNA or peptides within complexes will be matched in different samples, to determine the ordering of the components.

Principal Investigator: Professor Ian Hamley
Experimenter: Dr Valeria Castelletto
Experimenter: Mr Lucas Mello
Local Contact: Dr Leide Cavalcanti
Experimenter: Dr Emerson SILVA

DOI: 10.5286/ISIS.E.RB2210074

ISIS Experiment Number: RB2210074

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Ian Hamley et al; (2022): Solvent contrast variation study of DNA nanocarriers based on Transportan peptides, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210074

Data is released under the CC-BY-4.0 license.