ISIS Neutron and Muon Source Data Journal

Charbohydrate-mediated protein-protein interaction at membrane surface

Abstract: In Parkinson?s disease, the specific events causing neuronal disfunction and death are still elusive. We recently reported that the soluble GM1 oligosaccharide (OligoGM1) administered to neuroblastoma cells, has neurotrophic and neuroprotective properties. Molecular modelling suggests the formation of a stable trimeric complex between OligoGM1 and TrkA and NGF proteins, but the details of their specific interaction are not well understood. We are currently working at discriminating among possible scenarios and we here propose to detect by neutron reflection the possible direct interaction between membrane-embedded GM1 and the soluble portion of TrkA and NGF. A synergistic action of the two proteins will be also investigated. In a wider perspective, our results will define a new paradigm on the modality of interactions between gangliosides and proteins at membrane surface.

Principal Investigator: Dr Valeria Rondelli
Experimenter: Dr Julio Martín Pusterla
Local Contact: Dr Stephen Hall
Experimenter: Professor Giuliano Zanchetta
Experimenter: Dr Sally Mahmoud Helmy Abdalla
Experimenter: Dr Alexandros Koutsioumpas

DOI: 10.5286/ISIS.E.RB2210183

ISIS Experiment Number: RB2210183

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Valeria Rondelli et al; (2022): Charbohydrate-mediated protein-protein interaction at membrane surface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210183

Data is released under the CC-BY-4.0 license.